Biotechnology CBSE 12th Notes | Chapter-1 Recombinant DNA Technology |
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Recombinant DNA Technology e-Note
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Contents -
- Introduction;
- Tool of rDNA Technology;
- Marketing Recombinant DNA;
- Introduction of Recombinant DNA into Host Cells;
- Identification of Recombinants;
- DNA Library;
- DNA Probes;
- Hybridization Techniques;
- Polymerase Chain Reaction (PCR);
- DNA Secquencing;
- Site-directed Mutagenesis
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1. Introduction
Steps Involve in Recombinant DNA Technology
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2. Tools of Recombinant DNA Technology
2.1 Enzymes
2.1.1 Restriction Endonuclease Enzyme
RFLP [Restriction Fragment length polymorphic DNA]
2.1.2 Modification Enzymes
2.2 Cloning Vectors
pBR322 & pUC Vector
2.3 Host Cells
4. Introduction of Recombinant DNA into Host Cells
5. Identification of Recombinants (Transformed Host Cells)
5.1 Replica Plating
5.2 Blue-White Screening
6. Polymerase Chain Reaction (PCR)
7. DNA Hybridization
7.1 Southern Blotting
8. DNA Library
8.1 c-DNA Library
8.2 Genomic DNA Library
10. DNA Sequencing
10.1 Sanger's Method
10.2 Maxam Gilbert's Method
11. Site Directed Mutagenesis
CBSE 12th Biotechnology
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1. Instant Notes
2. Syllabus
3. Question Paper Pattern
4. Question Papers
5. AnimoVideo Tutorials
6. PowerPoints
7. Disscussion
8. Exams After 12th
9. Scholarships after 12th
10. CBSE Website
-----------------------
1. Instant Notes
2. Syllabus
3. Question Paper Pattern
4. Question Papers
5. AnimoVideo Tutorials
6. PowerPoints
7. Disscussion
8. Exams After 12th
9. Scholarships after 12th
10. CBSE Website